RealTGN™ cryopreserved human pluripotent stem cell-derived trigeminal neurons provides a valuable platform for studying craniofacial pain, thermosensation, and sensory neuron subtype biology.
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These hiPSC-derived trigeminal neurons exhibit high expression of TRPM8, NaV1.8, and TRPV1, core markers of thermosensation and nociception, with expression levels approaching or surpassing those seen in primary human DRG (hDRG).
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•1M per vial CAT# 6020M1
Identity of TTX-resistant NaV currents on SyncroPatch 384
Trigeminal neurons, matured 5 weeks, were assayed by whole-cell patch clamp on the SyncroPatch 384. The identity and properties of TTX-resistant NaV currents were assessed. A) Application of 500 nM TTX reveals TTXr currents which can be blocked by a NaV1.8-selective drug, VX-548 (30 nM). B) Averaged traces before and after application of vehicle or VX-548 demonstrate current stability and clear effect of VX-548. C) Box and scatter plot of % block indicates that TTXr currents can be blocked >50% in the majority of cells. Data generated in collaboration with Nanion Technologies.
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Bulk RNA-seq Validation of RealTGN™ Now Available
RealTGN™ neurons have been transcriptionally profiled by bulk RNA sequencing and benchmarked against primary human dorsal root ganglia (hDRG). While direct comparison to primary human trigeminal neurons is currently limited by the availability of public datasets, this analysis will be performed as suitable data become accessible. Principal component analysis shows strong lot-to-lot reproducibility and reveals elevated expression of key sensory markers, including NaV1.8 and TRPM8, relative to human DRG samples.
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Version 1.1
Updated 12DEC2025
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